A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays

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A simple method for demonstrating the giardia lamblia trophozoite.

INTRODUCTION Giardia lamblia is a flagellated protozoan parasite of man. Only 2 stages i.e. the trophozoite and the cyst forms are observed in the life cycle of Giardia. The Giardia infection is acquired from drinking water or by eating food which is contaminated with cysts. The symptoms of the Giardia infection are foul smelling diarrhoea, flatulence, steatorrhoea, etc. Stool samples from the ...

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The Giardia lamblia trophozoite contains sets of closely related chromosomes.

Size variations in homologous chromosomes from six Giardia lamblia isolates have been demonstrated. Four or five intensely stained (major) bands as well as a variable number of lightly stained (minor) bands are present in pulsed field gradient separations. Southern blot analysis with total chromosomal DNA as well as chromosome specific probes indicates that each minor band cross-hybridizes with...

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Giardia lamblia Transcriptome Analysis Using TSS-Seq and RNA-Seq

Giardia lamblia is a protozoan parasite that is found worldwide and has both medical and veterinary importance. We applied the transcription start sequence (TSS-seq) and RNA sequence (RNA-seq) techniques to study the transcriptome of the assemblage A WB strain trophozoite. We identified 8000 transcription regions (TR) with significant transcription. Of these regions, 1881 TRs were more than 500...

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Comparison of Giardia lamblia and Giardia muris cyst inactivation by ozone.

Inactivation of Giardia lamblia and Giardia muris cysts was compared by using an ozone demand-free 0.05 M phosphate buffer in bench-scale batch reactors at 22 degrees C. Ozone was added to each trial from a concentrated stock solution for contact times of 2 and 5 min. The viability of the control and treated cysts was evaluated by using the C3H/HeN mouse and Mongolian gerbil models for G. muris...

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Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro

Background and purpose: Antimicrobial peptides (AMP) are one of the most diverse antimicrobial compounds that have received much attention due to the development of drug resistance of pathogens to conventional antibiotics. But, few studies have evaluated anti-parasitic properties of AMP. The present study was conducted to compare the effect of a cecropin–melittin chimeric peptide (CM11) and met...

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ژورنال

عنوان ژورنال: BMC Microbiology

سال: 2011

ISSN: 1471-2180

DOI: 10.1186/1471-2180-11-91